Resultado da pesquisa (2)
Termo utilizado na pesquisa Ribeiro O.C.
#1 - Diagnóstico sorológico da brucelose bovina em animais adultos vacinados com dose reduzida da cepa 19 de Brucella abortus
Abstract in English:
ABSTRACT.- Jardim G.C., Pires P.P., Mathias L.A. & Ribeiro O.C. & Kuchembuck M.R.G. 2006. [Serological diagnosis of bovine brucellosis in adult herd vaccinated with Brucella abortus strain 19 reduced dose.] Diagnóstico sorológico da brucelose bovina em animais adultos vacinados com dose reduzida da cepa 19 de Brucella abortus. Pesquisa Veterinária Brasileira 26(3):177-182. Departamento de Medicina Veterinária, Universidade para o Desenvolvimento do Estado e Região do Pantanal (Uniderp), Rua Alexandre Herculano 1400, Parque dos Poderes, Campo Grande, MS 79037-280, Brazil. E-mail: gustavoj@mail.uniderp.br
The study evaluated the use of a reduced dose of the Brucella abortus strain 19 vaccine, in an adult herd negative for the disease, by serological diagnostic techniques, advocated by the Brazilian Program for Animal Brucellosis and Tuberculosis Control and Eradication, and by an indirect ELISA. The complement fixation test detecteed 46.77% positives, the rose bengal test 67.74%, the mercaptoethanol with standard agglutination test 87.09% and the ELISA ID 100%. The reduced dose influenced the serological diagnosis. None of the techniques reached a suitable specificity for use in the herd under those conditions, up to 3 months after vaccination.
Abstract in Portuguese:
ABSTRACT.- Jardim G.C., Pires P.P., Mathias L.A. & Ribeiro O.C. & Kuchembuck M.R.G. 2006. [Serological diagnosis of bovine brucellosis in adult herd vaccinated with Brucella abortus strain 19 reduced dose.] Diagnóstico sorológico da brucelose bovina em animais adultos vacinados com dose reduzida da cepa 19 de Brucella abortus. Pesquisa Veterinária Brasileira 26(3):177-182. Departamento de Medicina Veterinária, Universidade para o Desenvolvimento do Estado e Região do Pantanal (Uniderp), Rua Alexandre Herculano 1400, Parque dos Poderes, Campo Grande, MS 79037-280, Brazil. E-mail: gustavoj@mail.uniderp.br
The study evaluated the use of a reduced dose of the Brucella abortus strain 19 vaccine, in an adult herd negative for the disease, by serological diagnostic techniques, advocated by the Brazilian Program for Animal Brucellosis and Tuberculosis Control and Eradication, and by an indirect ELISA. The complement fixation test detecteed 46.77% positives, the rose bengal test 67.74%, the mercaptoethanol with standard agglutination test 87.09% and the ELISA ID 100%. The reduced dose influenced the serological diagnosis. None of the techniques reached a suitable specificity for use in the herd under those conditions, up to 3 months after vaccination.
#2 - Evaluation of an inactivated vaccine against caseous lymphadenitis of goats kept under extensive management
Abstract in English:
The experiment was carried out during a 2-year period (Oct. 1981 to Oct. 1983), using 163 goats of both sexes with 6 to 12 months of age, The objective was to evaluate the immunogenicity of a vaccine against caseous lymphadenitis, in northeastem Bahia. The vaccine was developed, utilizing the local strain 1002 Curaçá of Corynebacterium pseudotuberculosis isolated from na abscess of a goat. For the preparation, tryptose broth, enriched with yeast extract and lactalbumin, was used. Inactivation was effected with 0.5% formaldehyde, and aluminum gel phosphate was used as an adjuvant. The cell volume varied from 0.65 to 0.7%. The experiment consisted of the following treatments: A1 - animals vaccinated every 6 months; A2-animals vaccinated once a year; and A3 - animals not vaccinated. The goats were vaccinated by subcutaneous inoculation of 3 ml vaccine into each axillar region, and post-mortem examination was made at the end of the experiment. The results showed that 72.1% of the control animals (A3) had moderate to severe lesions in different lymphnodes, and in some cases in the internai organs. Of the vaccinated animals, only 25.9% and 18.9%, at 6 and 12 months interval respectively, showed discrete lesions which were restricted to no more than three lymphnodes per animal. There was a signif cant difference (P<0,05) between the vaccinated and the non-vaccinated animals, but no difference between treatment A1 and A2. It can be estimated that the vaccination gave a protection of 77% against the disease.
Abstract in Portuguese:
Foram utilizados 163 caprinos de ambos os sexos, de 6 a 12 meses de idade, com o objetivo de avaliar a imunogenicidade de uma vacina contra a linfadenite caseosa, no Nordeste do Estado da Bahia. A vacina foi elaborada com a cepa 1002 Curaçá de C. pseudotuberculosis, isolada de abscesso de caprino, cultivada em caldo tryptose, enriquecida com extrato de levedura mais lactalbumina e inativada com formaldeído a 0,5% durante 7 dias, utilizando-se como adjuvante o gel fosfato de alumínio. O volume celular variou de 0,65 a 0,70%. O experimento constituiu-se dos seguintes tratamentos: A1 - animais vacinados semestralmente; A2 - vacinados anualmente; A3 - animais não vacinados. Os animais vacinados foram inoculados via subcutânea com 3 ml da vacina em cada região axilar e, ao final do experimento, foram necropsiados. Os resultados mostraram que 72,1 % dos caprinos do grupo testemunha A3 se infectaram naturalmente e apresentaram lesão moderada a severa em vários linfonodos e, em alguns casos, nos órgãos internos. Dos vaciandos, apenas 25,9 e 18,9%, respectivamente, para os revacinados com 6 e 12 meses de intervalo, apresentaram lesões discretas que se restringiram a não mais de três linfonodos por animal. Houve diferença significativa (P<0,05) entre os animais vacinados e não vacinados mas não houve diferença entre os tratamentos A1 e A2. Pode-se estimar que a vacina proporcionou 77% de proteção contra a doença.
